Protection from Free β-Tubulin by the β-Tubulin Binding Protein Rbl2p

Rbl2p, a yeast protein that binds to β-tubulin and participates in microtubule function in vivo

Genetic configurations resulting in high ratios of beta-tubulin to alpha-tubulin are toxic in S. cerevisiae, causing microtubule disassembly and cell death. We identified three non-tubulin yeast genes that, when overexpressed, rescue cells from excess beta-tubulin. One, RBL2, rescues beta-tubulin lethality as efficiently as does alpha-tubulin. Rbl2p binds to beta-tubulin in vivo. Deficiencies o...

Computational study of binding of epothilone A to β-tubulin.

Understanding the interactions of epothilones with β-tubulin is crucial for computer aided rational design of macrocyclic drugs based on epothilones and epothilone derivatives. Despite numerous structure-activity relationship investigations we still lack substantial knowledge about the binding mode of epothilones and their derivatives to β-tubulin. In this work, we reevaluated the electron crys...

Pathway Leading to Correctly Folded β-Tubulin

*Department of Biochemistry noff et al., 1989; Ostermann et al., 1989; Martin et al., New York University Medical Center 1991; Gething and Sambrook, 1992; Gao et al., 1992; New York, NY 10016 Horwich et al., 1993; Lewis et al., 1996). †Flanders Interuniversity Institute for Biotechnology It has not provedpossible to obtain exchange-compeUniversity of Ghent tent tubulin following denaturation of...

Isolation of tubulin polyglutamylase from Crithidia ; binding to microtubules and tubulin , and glutamylation of mammalian brain α - and β - tubulins

A novel acetylation of β-tubulin by San modulates microtubule polymerization via down-regulating tubulin incorporation

Dynamic instability is a critical property of microtubules (MTs). By regulating the rate of tubulin polymerization and depolymerization, cells organize the MT cytoskeleton to accommodate their specific functions. Among many processes, posttranslational modifications of tubulin are implicated in regulating MT functions. Here we report a novel tubulin acetylation catalyzed by acetyltransferase Sa...